single cell mass cytometry  (fluidigm)

Journal: Bioinformatics advances

Article Title: Different approaches to Imaging Mass Cytometry data analysis.

doi: 10.1093/bioadv/vbad046

Figure Lengend Snippet: Fig. 2. A schematic overview of the Imaging Mass Cytometry workflow (created with BioRender.com)

Article Snippet: IMC was developed in 2014 based on earlier available suspensionbased, single-cell mass cytometry [cytometry time of flight (CyTOF)] technology [described in Bandura et al. (2009)] but combined with an additional platform for UV ablation (Hyperion Tissue Imager, Standard BioTools, South San Francisco, USA) and performed on stained tissue sections, giving the spatial resolution of the data (Fig. 1A) (Bandura et al., 2009).

Techniques: Imaging, Mass Cytometry

Journal: Acta Pharmaceutica Sinica. B

Article Title: Self-assembling, pH-responsive nanoflowers for inhibiting PAD4 and neutrophil extracellular trap formation and improving the tumor immune microenvironment

doi: 10.1016/j.apsb.2021.11.006

Figure Lengend Snippet: Effect of ZD-E-1 on tumor immune microenvironment. (A) Scheme of single-cell mass cytometry experiment. (B) t-Distributed stochastic neighbor embedding (tSNE) plot showing protein expression. (C) tSNE plot for immune cells from LLC tumor. (D) DCs, MDSCs, and CD4 T cell frequency (percent of CD45 + cells) in tumor tissue. (E) Expression of LAG3 protein in different cell groups. (F) Expression of CXCR3 and CCR4 protein in CD4 or CD8 T cells. (G) Effect of ZD-E-1 on immune cells and functional protein in tumor tissue. Data are presented as mean ± SD ( n = 3). ∗ P < 0.05 and ∗∗ P < 0.01.

Article Snippet: Lastly, we used single-cell mass cytometry (Fluidigm, Helios, San Francisco, CA, USA) to measure the population of immune cells and expression of immune-related proteins in LLC tumor tissues.

Techniques: Mass Cytometry, Expressing, Functional Assay

Journal: Journal of Hematology & Oncology

Article Title: Applications of single-cell sequencing in cancer research: progress and perspectives

doi: 10.1186/s13045-021-01105-2

Figure Lengend Snippet: Summary of important single-cell sequencing technologies and platforms

Article Snippet: HCC , Single-cell mass cytometry, RNA sequencing , Fluidigm , Revealed that the lncRNA HOXA-AS2 is associated with the regulation of cancer stemness during tumorigenesis and poor prognosis; identified an EPCAM + C-MYC + CK19 subpopulation in HOXA-AS2 high patients , [ ] .

Techniques: Sequencing, Single-cell Isolation, Library Amplification, Ligation, In Vitro

Journal: Journal of Hematology & Oncology

Article Title: Applications of single-cell sequencing in cancer research: progress and perspectives

doi: 10.1186/s13045-021-01105-2

Figure Lengend Snippet: Key findings related to malignant cell heterogeneity among tumors obtained using single-cell sequencing

Article Snippet: HCC , Single-cell mass cytometry, RNA sequencing , Fluidigm , Revealed that the lncRNA HOXA-AS2 is associated with the regulation of cancer stemness during tumorigenesis and poor prognosis; identified an EPCAM + C-MYC + CK19 subpopulation in HOXA-AS2 high patients , [ ] .

Techniques: Sequencing, Inhibition, Protein-Protein interactions, Flow Cytometry, Expressing, Next-Generation Sequencing, Phospho-proteomics, Transformation Assay, Mass Cytometry, RNA Sequencing